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Old 12-03-2006, 02:50 PM   #1 (permalink)
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D.J. Short articles

This thread is for articles written by DJ Short.
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Old 12-03-2006, 02:51 PM   #2 (permalink)
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Harvesting Marijuana
When To Harvest Your Marijuana Crop

By DJ Short

There are several important points to consider when choosing the optimum time to harvest your marijuana crop.
There are different factors to consider between indoor and outdoor marijuana plants. There is the difference between Sativas and Indicas in bloom duration and final effect.

There is the difference between early and late harvest to encourage head to body high respectively. There is the issue of chemistry because what we are really considering in terms of the ripeness relates directly to the chemical nature and state of the plant at harvest. Last, but not least, is the concept of the window for marijuana harvest, where I will begin.



Window For Harvesting Your Marijuana Crop

This term indicates the period during which the plant is at its optimum state of ripeness. The window opens when the plant is first ripe. Somewhere along the line the plant becomes over-ripe which signifies the closing of the window of harvest.

For most Indicas grown indoors, the window of harvest is about two weeks long give or take a couple of days for various strains. Indoors, if going directly from an 18/6 hour vegetative light cycle to a 12/12 hour bud cycle, most indicas take about eight weeks to fully mature.

As to outdoors I can only speak from experience at the 45th parallel and the bloom times there. At the 45th parallel I've found most Indicas to be at peak harvest starting at the beginning of October, sometimes very late September, and running until the end of October, sometimes running into November during mild years or in a greenhouse. My favorite time to harvest a nice Blueberry Indica outdoors is in the second to third week of October.

For Sativa grown either indoors or outdoors, the window may be open much longer. Some Sativas take up to thirteen weeks to mature indoors. Outdoors many will go well into November and even December, if conditions are right (again, this is near or at the 45th parallel). Head High or Body Stone?

An important consideration has to do with one's preference for a head high or more of a body high. A good head high can positively influence one's mental state much like a psychedelic; whereas a good body high is more similar to a narcotic effect. Generally, head highs tend to be more up and body highs tend to be more down. Suffice it to say that a good healthy mix of the two is a fine goal to achieve.

Sativas and early window harvests tend to be more of a head high, whereas Indicas and late window harvests tend to be more of a body high. Given this rule of thumb you can pretty much come up with what you want. That is, if you prefer a very psychedelic head high, then an early harvested Sativa might do best.

If a very narcotic body high is desired, then a late harvest indicas would probably do best. For that best-of-both worlds high, experimentation with late harvested sativas and early-to-mid harvested indicas usually proves interesting.



Magnify Your Buds

When we speak of various highs experienced by different products, we are noting variations in plant chemistry. The chemicals we enjoy are produced within the glandular stalked trichomes, along the surfaces of the bud flowers (calyxes), bracts, leaves and stems, starting in or around the fourth week of the bud cycle. More and more of these trichomes develop as the plant matures.

I highly recommend that the serious cannabis student acquire a 30X power, illuminated magnifier. These can be found at most local electronics stores, often for under fifteen dollars. With the aid of the magnifier one can learn more about the detail of trichome development and ripeness.

As far as trichomes are concerned, the tall ones with swollen, clear, bulbous heads are what to shoot for. The denser the concentration, the greater the potency.




The Cycle Of Marijuana Plant Maturity

Starting in the third or fourth week of the flowering light cycle, glandular stalked trichomes will begin to form along the surfaces of leaves, flowers, bracts and stems. At the same time, more and more flowers (also called calyxes) develop into densely-packed floral clusters.

The pistils of the young flowers are bright white and turn reddish brown with age. The pistils and flowers develop from the bottom of the bud to the top. The older, lower pistils are the first to turn reddish brown. For most basic indicas this usually happens by the sixth week in the flowering cycle. It is about this time that the calyxes begin to swell.

Calyx swelling is a major indicator of peak maturity. The lowest, oldest calyxes swell first and the swelling works its way up to the highest, youngest flowers on each bud. At peak maturity about 90% of the calyxes will almost look seeded, they are so fat.

Three quarters to 90% of the pistils will have turned reddish brown as well. For a basic Indica this takes well into the seventh week of the flowering cycle.

By the end of the eighth week most of the calyxes will have swollen and a surge of trichome development has coated most of the buds. It is now that the development of a very discerning palate comes into play to determine the finest harvest time. Remember - patience is a virtue and often a discipline.

The ripening signs for most Sativas are highly similar, except extended over a longer period of time. Occasionally, some Sativas have windows of peak harvest that actually open and close. That is, for a week or so the plant may exhibit signs of peak ripeness.

However, a week later the plant may have a growth spurt, which lowers the trichome-to-fibre ratio and overall potency for a little while. Usually a fibrous growth spurt is accompanied by a corresponding trichome increase. Again, time and experience are the key elements in this regard.




Changes In Marijuana Chemistry

As the plant matures through its window of harvest its chemistry changes. As the window closes, the more desirable compounds begin breaking down into less desirable ones. Primarily it is THC breaking down in CBNs and CBDs.

Which particular combination of chemicals is the most desirable is purely a matter of taste and choice, developed over time and with experience. Set and setting also play an important role in determining which type of product is best appreciated.

Pleasant head highs are often desirable for social occasions, whereas a narcotic late-window Indica may work better as an evening medicinal herb. The main point is that these differences are chemical in nature and more research is needed to more fully understand this phenomenon.

Another important point is that much can be done to further enhance the chemical process, especially in regard to bouquet, aroma and flavour, given the proper curing process.
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Old 12-03-2006, 03:03 PM   #3 (permalink)
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By DJ Short

General Irregularities/Anomalies of Cannabis Relating to Transgressive Segregation

Some of the Blue Family lines of cannabis (True Blueberry, Grape Krush, etc.) are known to occasionally sport various anomalies and irregularities. The main anomaly reported from these lines is that of the "krinkle" leaf type. Also referred to as a form of variegation this irregularity usually involves a twisting or convolution along half of the leaf divided along the central leaf vein. This anomaly usually affects anywhere from 5% to 20% of a given sample depending on the strain (TB = 5% to 10%, GK = 10% to 20%). In and of itself, this trait does not affect yield or the overall health of the plant. It is merely a simple deformity unique to this line of cannabis.

It is important to know the differences between simple deformities and more complex mutations. Simple deformities and anomalies are semi-common phenomenon whereas genetic mutation is by far more rare and profound. Most of the irregular expressions witnessed in some of the Blue Family lines are mere deformities. Very few are true gross mutations and those are usually sterile or non-viable (usually < %1).

I used to think that the deformities witnessed in certain lines of cannabis were strictly the result of a mutagenic regimen such as colchicine. Variegation in particular is a typical symptom of such a process. If such a process were used on the varieties of cannabis I have worked with, I assume that it was in the Thai lines as that is where most of these traits seem to originate. There is, however, another aspect to consider regarding these anomalies called transgressive segregation.

Transgressive segregation refers to the situation where the progeny from crosses of distinctly different P1's exhibit characteristics beyond what either parent exhibited. A good botanical example is that of the cabbage family from which broccoli, cauliflower and Brussel sprouts developed from the same meager beach cabbage. This is the level of the diversity witnessed in the f2's and beyond crosses of the plants that I have worked with.

The combination of the two very pure, unique and distinctly different varieties of indica and sativa resulted in the extreme variation in the f-2 and beyond progeny. This is where the anomalies, irregularities, eccentricities and effects of transgressive segregation are witnessed and isolated. It is from this extended diversity that new and exciting lines are discovered and isolated. Simple leaf irregularities are no reason to abandon a particular line, and perhaps are an indication of something worthwhile. Suggesting that these anomalies are in some way detrimental to the overall cannabis gene-pool is akin to suggesting that broccoli, cauliflower and Brussel sprouts are in some way detrimental to the cabbage family.

[Side Note: What may have been very detrimental to the overall cannabis gene-pool was the "willy-nilly" introduction of indica and indica-based genes to the equatorial regions of the world?circa 1980's. The tropics are susceptible to aggressive takeover by dominant species and sub-species. This may be what happened to the fine sativa land races of yore?coupled with human selection for production-based indica varieties. Hopefully this phenomenon will be relatively short lived as human selection re-kindles the near-lost memory of the beloved land-race sativa.]

The structural differences witnessed in some of the Blue line individuals including anomalies such as leaf shape, leaf krinkle and color variations, along with basic aesthetic qualities such as range of flavor and palate, issues of duration, tolerance and even shelf-life are more than likely the results of transgenic segregation.
None of these minor abnormalities are in any way detrimental to the overall gene-pool. There do occur very rare instances of freakishly deformed and stunted individuals. Every one of these I have ever encountered has been sterile or non-viable. However, some have produced some of the most unique finished product I have ever sampled in terms of flavor, potency and effect. Unfortunately, these extreme abnormalities are not only rare and sterile, most are also nearly impossible to clone.

Please remember, my number one goal when breeding fine herb is that of the finished product. All other factors; structure, color, growth rates and patterns, leaf shape, odor, flavor, height, flowering characteristics, even potency (please see my other comments concerning bland potency versus exciting quality in my book and other articles), etc. are of lesser concern. My secondary concern is the minimization and elimination of hermaphrodites. This is primarily for the indoor community's behalf, as it is my belief that true breeding equatorial (outdoor bred and produced) strains are all monoecious to some degree. It is the quality of the finished product that I aim for with all of my work. Structural considerations are selected for only after a variety passes the "head/body" test.

Please note also that I consider myself more of an artist than a scientist. I respect science for what it is (discipline and controlled focus) and enjoy its utilization, but for me it is still simply another tool with which to create. With that said please allow me to state that much of the science (definitive recipe) involved in supporting my goal (to discover, create and produce truly good herb) remains somewhat of a mystery to me. Due to all of the truly good herb I have had the good fortune to sample, I am confident in identifying what I consider to be truly good herb?with or without the science. I am equally curious, as are many, regarding the scientific observations, disciplines and discoveries that support this goal.

Toward that goal there are a few points I would like to attempt to clarify:

The Purple Thai. As mentioned before, the Purple Thai was a cross between an outstanding Highland Oaxaca and a very freaky Chocolate Thai.

The Chocolate Thai was one of the most difficult plants to grow. It was dark and very asymmetric, rarely sporting any kind of a definite main-stem for any length of time. It was difficult to clone and very hermaphroditic, producing small airy buds at best. The herb from the Chocolate Thai passed the test (the progeny were at least as good as, if not better than, their parent), but had certain problems otherwise (structural and hermaphroditic). It was also similar in potency to the Highland Thai regarding strength, length and type of high.

The Highland Oaxaca was structurally a different strain (tall, with a definite main-stem, symmetric with less hermaphroditism and better formed buds). The two were crossed and the resulting seeds produced the famed Purple Thai that I was able to use for quite awhile. The Purple Thai exhibited the symmetry and desirability of the Oaxaca with the potency and dark coloration of the Chocolate Thai?the best of both worlds, and its hermaphroditism was much more manageable. It also cloned well. The plants from these seeds also passed the test of equal or improved quality progeny in terms of finished product. Personally I preferred the finished product of the Purple Thai to the Chocolate, not just due to its growth patterns but in terms of the overall effect. The original Highland Oaxaca produced the most desirable finished product, in my opinion, and has yet to be replicated to my complete satisfaction.

So technically the Purple Thai was not a true, land-race acclimated P1. However, as far as I was concerned it fit the bill quite well in that it was very consistent in the sativa genes it carried and passed (nearly identical to the Highland Thai). It was also the closest to producing the desirability of the Highland Oaxaca?s finished product.

The Highland Thai, could possibly have been from Burma (now Myanmar), as borders between the two countries are questionable and change. Other drainage systems in the region lead to the Eastern Himalaya, meaning that the "Highland Thai" could have originated from a number of places. The entire Himalayan region has perhaps the greatest concentration of geographic "sweet spots" in the world, many worthy of exploration. The Highland Thai grew much like the Chocolate Thai; asymmetric, with more stretch?especially in the bud structures.

The difference in the finished product between the Highland and Purple Thai was that the Highland Thai was more potent while the Purple Thai was more "kind" ("kind" being more comfortable to endure, especially while tripping). Another way to put it is that the Highland Thai could be more "racy" or "scary" while the Purple Thai tended to be more "relaxed" or "happy". Both had no ceiling or noticeable tolerance effect, and both provided a long lasting experience, as well. Both also shared a very similar sweet/fruity flavored phenotype that was unmistakably Juicy Fruit/Tutti Fruity?the ultimate female breeder (Note: this was from the plants grown locally--Oregon, indoor and out).

Regarding a clue to the equatorial "Holy Grail" such as the Highland Oaxaca and Santa Marta Gold (South and Central American varieties) a certain, unmistakable flavor comes to mind: that of incense cedar/frankincense/burgundy/floral with spicy/savory undertones. Coffee, chocolate and fine tobacco were also present, but the sweet incense cedar is what I most recall from the Highland Oaxaca and Colombian Gold varieties. The Thai was more complex with more spicy/savory aspects atop a finely distilled burgundy (probably more from the cure) and sort of a "dying" floral, sickly-sweet aroma that was unmistakeably Thai herb (sometimes from Hawaiian as well).
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Old 12-03-2006, 03:03 PM   #4 (permalink)
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Part 2

An important aspect to note here is that, according to the best of my knowledge, most high quality sativa of the era was in some way shape or form of Thai origin (or greater Himalayan, but for simplicity?s sake referred to as "Thai"). This includes the Oaxaca, Colombian, Panamanian, Hawaiian among others of the late 1970's. Most of it supposedly came from Thai seed stock. Having sampled many of the region-of-origin varieties of the time, I must concur. Many of the Central, South American and the great Island herbs of the time were more than likely of Thai origin. Similarly, today?s version of ?Sweet Skunk? (a misnomer for sure) when grown to fully finished bloom and with a long-time cure shares a distinct Thai quality as well.

Therefore, it seems Thai was the fundamental building block for most quality commercial sativa of the 1970's. ("Commercial" is a key word in the previous sentence as I am sure that there were/are many different origins of the regional high quality herbs.) Certain aspects of acclimation must have been responsible for the subtle (and not-so-subtle) differences of the various herbs of the time.

My definition of acclimation is: grown in a specific region or circumstance for enough generations (in-line bred) to impart characteristics unique to the region or circumstance?prefera bly in an herbal "sweet-spot" such as Highland Oaxaca. This is a very important consideration regarding future resurrection of land race varieties.

Side Note: When I was working with these sativa back in the 1970's it was all done either outdoors or under large florescent systems, 8' bulbs set onto 4'X8' sheets of plywood and suspended from 2"X4" frames (obviously before the advent of HID lighting). This was also before the introduction of the Indica (Afghan) genes, which appeared commercially at the same time as HID lighting (circa 1978-?79).

The indica, or "Afghan" varieties became commercially available at the end of the ?70's, and were the polar opposite to the Thai?s and other sativa. It is interesting to note that Afghanistan is west and north of the Himalaya while Thailand (and Burma) is east and south. Short, stout, wide-leafed, very symmetric and adapted to a short flowering cycle, the indica is what brought the musky "skunk" odor to the game. I must say that I was not a big fan of the pure indica "skunk" strain. It is the primary culprit responsible for of much of the couch-lock characteristic found in much of today?s herb. Indica usually has a low ceiling and a quick tolerance buildup. In short, it is boring, bland and dull herb, unless one seeks anesthetization (or hashish production).

In the indica?s defense it needs to be noted that it helped birth the indoor cannabis industry. Without the indica?s fast flowering cycle or its dense bud production or its short stature, the indoor grow scene would be very different today. It was somewhat obvious to recognize indica?s virtues and liabilities for what they were. It was work and dedication to breed out the bad and to strive for the desirable. The primary virtue of the indica variety is that of its contributions toward hashish production.

An interesting side-note to the indica breeding scheme was the initial direction of the cross. I found much better success with the sativa/indica cross (the female sativa crossed with the indica pollen) than with the opposite indica/sativa cross (the female indica pollinated with the sativa pollen). Again, this was primarily for quality of finished product from the progeny. I found my "Holy Grails" via the diversity of the sativa/indica cross.

Other anomalies witnessed from the extreme diversity of cannabis include examples of polyploid expression?triple and quadruple types, twins?including various forms of conjoined twins, double leaf and double root phenomenon, along with extreme variation in odor, color and flavor, etc.

So the successes of transgressive segregation are highly dependent upon the selections made in the P1 and f2 generations. These are the most important generations regarding overall selection. The direction of the P1 cross also seemed to play a part in successfully finding high quality herb.

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Old 12-03-2006, 03:10 PM   #5 (permalink)
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By DJ Short

Choosing your parents

The place for breeding to begin is with choosing the parent plants, called the P1 generation. For best breeding results you use true-breeding stabilized strains as your P1's. Different breeders have different standards as to what qualifies as a P1. I have very high standards for my P1 generation. For me, the P1 must be either a fully acclimated, region-of-origin land-race variety, or no more than one generation removed, and crossed with itself or another highly similar, region-of-origin land-race variety.

I used three P1 strains to breed Blueberry, Flo and others. They were the Highland Thai (also called Juicy Fruit Thai, a first-generation Thai seed grown in the Pacific Northwest); a cross called Purple Thai which was a first generation land-race Chocolate Thai crossed once with a first generation land-race Highland Oaxaca Gold; and an Afghani Indica which came to me one generation removed from Afghanistan via the California/Southern Oregon growing community.


The Highland Thai was a joy to grow and behold, despite its hermaphroditism. This plant grew fast, filling in any empty spaces with lush, green growth. It was a very slow finisher, 12 to 16 weeks and beyond in the bud period for most. It had the longest and skinniest leaves out of all the plants I have worked with. Thick side-branching is another characteristic of this variety.

The plant only periodically produced any kind of "tight" bud structure. Most of the buds were very loose, with some sporting long, slender shoots of widely-spaced single female flowers in a row (especially when grown hydroponically under halide lights.)

This bud structure is known as "spindly". Many of these spindles resemble threads protruding from a semi-formed bud. Each single thread averaged anywhere from five to ten inches long, some even longer, and consisted of a row of evenly-spaced female flowers and their corresponding bract leaves, anywhere from a quarter inch to one inch apart, alternating bract and flower in single file.

The entirety of the "thread" and bud structure was coated with sweet/fruity aromatic resin glands.

The overall plant color was dark, while the bud structures matured a lighter shade of green, sometimes green/yellow.

I was never able to get a Juicy Fruit Highland Thai to "over mature". I took one to almost twenty weeks into its flower cycle and she just kept pumping it out. Outdoors, one was taken in early-mid December from a greenhouse. The only difference was that the later harvest was a more stony, body high.

The finished product from the Highland Thai was an all-around champion herb. Though difficult to trim and cure, the outcome was fully worth the effort. It was a powerful, long-lasting and exquisitely flavoured herb with little or no ceiling. The high could last up to seven hours! The flavour, aroma and taste were a totally sweet tropical punch ? tutti-fruity all the way.

The Purple Thai was the other sativa in my repertoire. This was a first generation cross between the Highland Oaxaca Gold and the Chocolate Thai. This cross grew medium/tall and was very symmetric in structure. The side branches were shorter and, if left alone (untopped) the main stalk (meristem) remained the dominant shoot.


The entire plant of the Purple Thai was very dark-coloured and would express a deep royal purple colour at the slightest exposure to cold. It did not exhibit any of the spindly bud syndrome of the Juicy Fruit Thai, and the finished buds were a medium and compact sativa type. The finished product was equally as fruity and strong as the Juicy Fruit, also without ceiling.

For whatever aesthetic reason, I preferred the Purple Thai to the Juicy Fruit Highland Thai. I believe that the Purple Thai was emotionally kinder or gentler than the Juicy Fruit. At larger doses the Juicy Fruit could evoke quite a terror, especially when combined with psychedelics. Though no less potent, the Purple Thai seemed easier to handle, including when tripping. The Purple Thai was one of the first to show resin gland production in the early bud cycle, at roughly three to four weeks into the cycle. It also matured at 10 to 12 weeks indoor, and early to mid November outdoors.

The Afghani Indica plant is short with large, wide leaves, stout and thick-stemmed. It has early to very early maturation, producing large, dense buds that smell earthen to skunk, with a strong smoke that is generally sedative or "down" in effect. Though consistent in its growth and overall effect, its appeal is somewhat limited in my opinion. I believe more indicas should be made into hashish, which is where the finer qualities of the indica appear.

The sinsemilla Afghani Indica first showed up on the market in 1979. They were huge, green, stinky, sticky, dense buds of potent, pungent herb that smelled like a skunk and produced a narcotic-knockout stone that was tremendously novel, when compared to all the sativas that had come before. This was right after sinsemilla herb hit the market with big appeal.

The triad of sinsemilla, indica, and the advent of high powered halide and HPS lights, all wreaked havoc on the breeding programs of most pot-entrepeneurs. Few people maintained their sativa lines, and the strains virtually disappeared from the commercial markets. The short, dense, early-maturing and body-powerful indica has dominated the scene since 1983 ? a matter of disjointed economics.

Such were the three main P1's I used for my breeding lines.

The f1 cross

The f1 cross is the first cross between two distinctly different P1 parents. The "f" stands for filial (child). I cannot overstress the importance of the two P1 parents being as genetically different as is possible. It is this initial genetic diversity that leads to the most possibilities in succeeding lines.

If the P1's are sufficiently diverse, then the f1 will be a true hybrid, expressing a near total uniformity and great vigor. It is in the crosses beyond the initial f1 (especially the f1xf1=f2 cross) that specific traits are sought. There will be a tremendous amount of variance in the f2 crosses of f1's obtained from a female pure sativa and a male pure indica.

The Blueberry (among others) was discovered and stabilized from an f1 cross between the P1 parents of a female Juicy Fruit Thai or a female Purple Thai and a male Afghani Indica. Thus there were two possible routes to essentially the same finished product. Blue Velvet and Flo seem more accessible via the Purple Thai route, while Blue Moonshine seems more accessible through the Juicy Fruit lineage. That is, there is a higher probability of occurence of the specific traits which I'm seeking, and so they're easier to "find".

Oddly enough, the opposite cross (female Afghani indica crossed with pollen from male Thai sativa) was not nearly as interesting. The f1's from this cross were more leafy and less desirable. They were also more hermaphroditic and subsequent breeding revealed them to be less desirable. It has been my observation that in a successful cross, the (usually female) sativa contributes the type of aroma and flavour, while the (usually male) indica contributes the amount of aroma and flavour to the prodigy. So far this observation has proven fruitful.

So the Thai female is pollinated with the Afghani male and an abundance of seed is produced. The seed is uniformly sized and shaped; small, ellipsoid and mottled with dark stripes upon a grayish brown shell. A single female is capable of producing thousands of seed, leaving plenty for experimentation. This is the f1 generation, which I called simply "The Cross".

The plants of The Cross grew uniform, medium-tall "spear" structures of many competing side-branches around one main (meristem) stalk. Large, long buds formed along the branches. There was a wide palate of colours, especially among the Purple Thai cross. The buds were lighter, almost yellow to the centres, wile the outer leaf, bract and calyx tips showed red, purple and blue hues. The maturation rates were uniform as well, with a wide window of harvest being between weeks eight to eleven in the bud cycle, indoors. The finished bud had a very strong "astringent" chemical/terpene aroma that bordered between pine, gin, licorice and paint. Only a very few of The Cross expressed hermaphroditism, about 1 out of every 25 females.
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Part 2

The f2 cross

The f2 is the second filial generation, simply a cross between any two of the f1 stock. With my f2 crosses the outcome was extreme, with almost every characteristic of the cannabis plant being expressed in some of the plants. The diversity was spectacular, both in structure and aesthetics. From sativa to indica, short to tall, dark to light, early to late maturation, wide to narrow leaves, along with an extensive array of flavours, aromas, tastes and highs. The f2 seeds collected were equally diverse, ranging from large to small, plump to slender, striped to solid, round to oval.

A grand amount of time, energy and money was spent from this point to isolate and stabilize the desired traits. There is a tremendous amount of work between the f2's and the f4's and f5's. Trial and error is the rule; certain paths prove futile while others bear further examination. On average, there are about nine errors to each success. Coupled with the difficult clandestine aspects of the trade through the 80's and 90's, it was a difficult task to accomplish. Many sacrifices were endured by my family and friends.

It was however, a fun and worthwhile occupation to sample all the research material. It was hard work and dedication to record the findings and attempt to create useful categories and find patterns and traits to specific characteristics. Then there's the wait for the cured sample. If the sample passed "the test" then the plant was kept for further consideration. The most desirable samples were used for further breeding to f3, f4 and f5. The harvested plants, cut above the lowest few nodes, were placed under a vegetative light cycle to stimulate new growth for cloning.

I like to do one backcross somewhere between the f3 and f5 generation. Exactly when, where and how that is done remains a trade secret for now. Another trade secret is the art of selecting the best males for breeding. These topics and others will be covered in future articles.

Select the best, reject all others

Mendelian procedures are fine for sweet peas, but when it comes to herb I much prefer Luther Burbank's philosophy: "Select the best and reject all others!" This simple phrase is worth much consideration. Mendel's work is useful, especially concerning P1 and f1 crosses. But beyond the f2 and f3 cross, Mendel's theories add copius complexity to the equation.

Your friend the freezer

A benevolent tool in our trade is the refrigerator and freezer. The fridge is extremely useful in extending the longevity of seed and pollen. The trick to successful freezing is to freeze deep (-10 to -40°F/-20 to -35°C) and then keep the seed undisturbed. Hard frozen objects are very fragile. The slightest shock may shatter crucial, delicate cell structures within the seed. Double wrap the seed in paper; little manilla envelopes work great.

I like to do small amounts, in one-time-use packets, to keep waste to a minimum. Then place the wrap into a plastic freezer bag, then place the freezer bag into a plastic tub or tupperware container. Now the seed is ready for the deep-freeze. In the fridge, storing seed in airtight, brown glass jars with a little rice or other non-toxic desiccant seems to work best.

I have had pollen last for years in a deep freeze. It must be frozen immediately after fresh collection from the plant, in as low a humidity as possible (preferably 0%). I like to shake the productive male flowers over a flat and clean piece of glass. The pollen pile is sifted to rid the unwanted plant material from the pure powder.

It is also useful to cut pollen with flour to stretch the amount. A pollen-to-flour ratio of 1:10 or even 1:100 works best. The cut pollen may then be separated into small, one-time-use amounts, stored in a flap of paper and frozen the same way as the seed. The frozen pollen must be applied to the live female flower immediately after thawing to increase viability.

The sweet sativa room

I recommend the creation of a special "sativa room" for indoor breeding of such strains. This room needs to consider and satisfy the unique needs of the sativa variety. The goal is to replicate the equatorial conditions of the world?s various "sweet spots". Some of these conditions include: a different light cycle than the standard 18/6 vegetative 12/12 bud cycles, a higher angle of light (using a straight track shuttle instead of a circular one), humidity control set on low for the highland and high for the lowland, and variations in soil composition and depth.

Light cycle is one of the key considerations for those wishing to breed truly fine quality cannabis indoors under lights. The 18/6 veggie and 12/12 bud cycles are perhaps the main influence towards the indica dominant strains and generic blandness of the indoor commercial product. A true equatorial sativa will require closer to a 13/11 vegetative and a long (four to six month) 11/13 flower cycle. Different variations may be tried, such as 15/9 veggie and 10/14 flowering cycle. Be prepared for much fine tuning.

Equatorial strains also experience a higher arch of sunlight than those grown beyond 38° north or south ? with a sunrise almost due east and sunset nearly due west. Therefore the sativa room will edintense overhead lighting with a straight track mover. Keeping the plant in a stationary position, especially through the bud cycle, may positively influence the outcome of the finished product.

As jungle (lowland) herb requires only a thin layer of nutrient soil, perhaps a four-to-eight inch layer of soil over clay or concrete (with some form of drain system) would encourage lateral root growth, stationary plants, and a more lowland sativa-friendly environment.

If successful, the sativa-friendly room can be used to acclimate an indoor sativa variety, which expands the possibilities of your breeding operation.
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Old 12-03-2006, 03:21 PM   #7 (permalink)
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By DJ Short

My Cataloguing System

c. 2004

Perhaps one of the most useful devices used in a quality cannabis breeding project is that of catalogue techniques. This refers to the method used to categorize various traits for future reference, or how to best label traits from a given population. It is also a means to track who came from where (generational references).

First and foremost, I cannot begin to describe the level of complexity involved with a breeding project that extends from the f-2 to the f-5 range. It took me over a decade and a half of trial and much error to fully comprehend and develop a system that actually works to this level and beyond. It starts out simple enough, until the f-2's, then the complexity expands exponentially with each generation.

The P-1's are simple enough, they are the original breed-stock and labeled for what they are, i.e. Highland, Purple or Chocolate Thai, Oaxacan or Santa Marta Gold, Pure Afghan, etc. The f-1's were equally simple as they were of uniform expressions and I simply chose to label them ?The Cross?. The f-2 generation was equally easy to identify with the label ?Double Cross?, or the progeny of the f-1 cross. However, when the f-2's were grown out, extreme diversity ruled the making of the f-3's (or the descriptions of the f-2's selected to breed further with) a tougher call to make.

It is at this level (and beyond) that some form of labeling system becomes necessary to catalogue all of the different variations found. Beginning with the plants grown out from the f-2 seeds I chose to utilize an alphabetized system with each letter corresponding to a specific trait. For example, the letter ?B? came to signify the ?Berry? characteristic, ?F? stands for ?Fruity? (sometimes ?Floral?), ?G? is for Grape, ?C? for Citrus, ?O? for orange, ?L? for lemon or lime, ?K? equaled ?Kush?, ?S? for ?Sativa? ?P? for Purple, ?X? for extreme glandular trichome production, etc.

I must confess that it took much trial and error to finally get it right. Therefore, if one were to look at my early notes many exceptions to what developed as ?the rule? can be found. I left these early ?mistakes? as they were so as not to over-complicate what came next. It is also very important to note that most of these observations were relatively subjective and that no more than two traits, or characteristics were ever assigned to any one plant. Therefore, the label ?BK? came to stand for ?Berry Kush?, or a Kush dominant plant with outstanding berry attributes. It is also important to note that only the most outstanding plant of any given attribute was selected for future work. So the plant that ended up with the ?BK? label was the most Berry-Kush of the lot.

So, my f-3 stock became labeled with a two-letter code indicating what the most outstanding characteristics of it?s parent (primarily mother) were, and only those with the strongest expressions earned their label. When the f-3's were grown out and crossed to make the f-4 generation, these labels were coupled to indicate the parents of the f-4 progeny, i.e. BK/FS would be a cross between an f-3 Berry-Kush mother (I always list the female first, male second with a back-slash in between) and an f-3 Fruity Sativa father.

F-4's and Beyond

Consider the label number: 4/5 3 96-2. This is the type of numbering symbol I use to label F-4 and beyond plants. Before we dissect this number I need to point out a few rules that I follow in a breeding project beyond the F-4 generation.

First, I only grow out no more than six varieties at any single time. The reason is to avoid too much confusion. Six is about the maximum number of varieties an individual can realistically keep track of. These six (or five, or four etc.) varieties are then labeled as ?1" through ?6" (or the number of varieties used). Let?s say the 6 f-3's I use are: 1. ?FK/FK?, 2. ?BK/PK?, 3. ?FK/FL?, 4. ?GK/GK?, 5. ?PK/FP? and 6. ?XP/FK?. Notes are made to record this fact and the seeds are then sprouted and grown using these simple, single digit identification numbers (1 through 6 in this example).

Second, I select only one male from any single breeding project. Again, this simplifies things and avoids mistakes enormously. That male is generally selected at about the third week in the flowering cycle, unless it is a clone from another project. After the single male is selected the other males are removed and the remaining females are numbered according to their variety category (i.e. if there are seven #1. females, five #2 females, etc. they are labeled #1?1 through 7, #2?1 through 5, etc.) The male simply retains the number from its variety label, in our above example the number ?5" (in the 4/5), or the ?PK/FP? male.

Now we may examine the above example: 4/5 3 96-2. The first two numbers, ?4/5" are the variety number of the female first and male second. So in this case that would be: a ?GK/GK? female crossed with the ?PK/FP? male. The third number in our example, ?3" means female #3 from the #4 (?GK/GK?) batch. The next number in the example, ?96" is merely the year and the final number is the crop number for that year. So, translated, the number 4/5 3 96-2 is the third ?GK/GK? (or #4) female crossed with the ?PK/FP? (or #5) male grown from the second crop of 1996.

Please note that the ?/5? male-used indicator will be /5 for all of the seeds labeled from this batch as the #5 (?PK/FP?) male is the only one used. If a male clone from a past crop is used it may be indicated by using the #7 in the initial notes (if six varieties are sprouted) and described as the male-clone-used in the #7 description. Likewise, if any of the six varieties tested are from a past clone (female), they may be selected as one of the #1 through #6 varieties, labeled and described accordingly.

It seems complex at first, but I assure you that it works great. The same system is used for the F-5 generation, and beyond. The system merely requires that dated notes be kept and catalogued. That way, any crosses may be backtracked and referenced via one?s notes and a simple, six or seven digit code is all that is needed to label and catalogue one?s plants.

Finally, this system works best for forward crosses mainly. Backcrosses will need another connotation to note their use . The ?clone-used? labeling described prior works well for backcrosses involving clones.

This system is good for only one grow out at a time. If multiple grows, or facilities are used then they will need to be noted as well, perhaps with a lettered ?A?, ?B?, ?C? etc. appended onto the catalogue number. Also, detailed notes of each individual plant are necessary to fully utilize any cataloguing system and are obviously required for success. Other than that, I have found this to be a relatively simple and foolproof system for cataloguing one?s breeding projects beyond the f-3 generation.



Background, Review and DJ?s Law

Remember; all of my seed-stock came from the cross of two distinctly different P1 parents with the mother being of pure, land-race sativa origin and the father being a pure indica. This cross produced a very uniform line I?ve referred to as ?The Cross?, or f1 generation. When ?The Cross? was bred with itself (dubbed ?Double Cross? at the time) the resulting variance was phenomenal in the f2 generation expressions. Beginning with this f2 generation, intense scrutiny and application of the selection rules and laws come into play. The bulk of the variation from this f2 generation were primarily discovered in the 1980's.

I must comment here that the variation witnessed from this f2 cross, and subsequent crosses, was truly amazing in its complexity of variance. I also need to mention the fact that, as far as ?the number?s game? is concerned (selecting from as large a population as possible), this f2, and to some degree the f3 generations are the most relevant. That is, the larger the number of f2's and f3's sprouted, the greater the degree of variance that is witnessed. It is from the f3 and beyond generations that specific traits are bred for and stabilized. Once a specific trait is recognized, the numbers necessary for success diminish with each generation successfully crossed toward the desired traits. In simple terms; the more f2's and f3's sprouted for examination the better. However, once a specific trait presents itself and is chosen for future work and appears to breed true through subsequent generation, the less f4's, f5's etc. that are needed to witness the desired results.



There is one very simple rule that I feel is primary when considering one?s involvement in a quality cannabis breeding project, or when applying Luther Burbank?s law (?Select the best and reject all others.?). It is an extension of Luther Burbank?s Law that I will refer to as:
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Old 12-03-2006, 03:21 PM   #8 (permalink)
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Part 2

DJ?s Law of Quality Cannabis Breeding.

?The progeny must equal or surpass its parent in overall quality and desirability to be considered for future breeding.?

That is, if the progeny is not as good as the bud it came from, it is rejected from further breeding. The finished product from the grown seed does not need to be exactly like the bud or parent from which it came. A good example is from the land-race Thai and the plants grown from its seed. The plants grown from the land-race Thai seed, especially produced indoors, were not much like the imported Thai from which it came (primarily due in this case to very different growing environments and curing techniques). It was, however, very equal, and in some instances superior to the buds from which it came and therefore worthy of consideration.

On the other hand, I have not had much luck in equaling the effects of certain tropical Island herbs such as Hawaiian or Jamaican indoors, and therefore these offerings never made the grade. For the record, the majority of land-race varieties grown out prove to fail DJ?s law, IMHO. Very few end up being of significant value or worthy of future consideration. But DJ?s law also applies to the selection of the f2's, f3's and beyond.

I realize that it is sometimes impossible in the current seed market to be able to sample a true example of the bud (parent) of the seed one purchases. Sometimes these varieties are commercially available in places such as a Dutch coffee shop, but one is never really certain if the bud one is purchasing (or the seed for that matter) is the real deal. This is perhaps one of the main flaws in the current seed market?reliability. Given this situation, the seed buyer and breeder will need to employ Luther Burbank?s Law first, and DJ?s Law after a parent is created for testing.

A Word About Mutagens

I am aware of concerns involving mutagens such as colchicine and their possible use on cannabis plants. Colchicine is a chemical that when applied to seeds or sprouts can cause extreme genetic mutations in future generations of the seeds that survive the treatment (often less that 1%). For the record let me state that I have never used colchicine, or any other mutagen, in my breeding work . All of my selections are from organically produced crops. I do have my suspicions, however, primarily concerning some of the Thai strains that I have used.

I am not certain, but I suspect that the Highland and Chocolate Thai may have been the results of a mutagenic regimen. The reasons I make the speculation is due to observations witnessed in the growing cycle of the Highland and Chocolate Thai and their progeny. Both were extremely ?freakish? in some of their expressions, as were a number of subsequent generations. These freakish anomalies are similar to many of the abnormalities documented by mutagenic experiments published in journals such as High Times and Cannabis Culture. These abnormalities include asymmetric growth patterns, ?albino? mutations that affect parts of the plant such as half of a leaf, various polyploid expressions and mild to extreme leaf mutations. I am very interested to learn about any first hand experience anyone may have had in this capacity. Having said that, one of the most important aspects to consider in regard to a breeding regimen is that of ratios.

Ratios

The math for this selection process involves watching the ratios of desirable plants from f2 to f3 and beyond generations. The ratio of plants exhibiting a specifically desired trait from the f2 generation may be 1:20 or 1:50 or 1:100 or even as high as 1:1000 (approximate ratios). Once obtained and selected, however, and crossed to the correct pollen source, this ratio will equate more and more per each successful generational cross. This is another indicator of which individuals actually breed true for the specific desired trait(s). Therefore, if the ratio of plants with desired traits presents itself in an approximate 1:100 ratio in the f2 generation, and successful crosses are made, this ratio should diminish to between 1:50 to 1:20 for the same desired trait in the f3 generation. If the cross remains successful, the ratio will diminish to anywhere from 1:10 to an absolute IBL (In-Bred Line) beyond the f4 cross of 1:2 (or 1:1 barring male sexual exclusion, i.e. the ratio among the female plants only).



It is important to note that any 1:2 (1:1 female) IBL ratio is generally for a very specific, singular trait. When considering combinations of traits, the best obtainable ratio I have found is between 1:5 to approximately 1:10, depending on the number of desired traits sought. Please note that these ratio numbers are approximate and the true numbers may be closer to the powers of two such as 1:8, 1:16, 1:32 etc. It also needs to be noted that my ratios relate to total number of seeds sprouted and not just the number of female plants.

Therefore, if I sprout 100 f2 seeds and find one female plant with any number of desirable qualities, and I successfully find a male f2 pollen donor to cross with, and the ratio of these same desirable plants in the f3 generation becomes at least 1:50 (preferably 1:30 or better) then I consider myself on the right track and proceed from there. If a subsequent cross of the f3's provides a ratio of desirability in the f4's of 1:20 (or closer), I am definitely on the right track. In essence these are the (general) numbers I look for in the early breeding trials. Suffice it to say that my informal observations have proven true enough for me to be able to judge desirable results with adequate success, despite the approximations.

Suffice it also to say that I have a large collection of f3's and f4's and beyond that merit further investigation. These f4's (and some f3's and f5's) are the primary source for all future breeding work along the lines established by the ratios of plants with the desirable traits expressed therein.

A Word About Anomalies

Anomalies, individuals that are markedly different from the general phenotypic expression of a given variety, are rare, but occur with a near predictable ratio. Beyond the f-3 generation (and from my personal seed-stock) anomalies present themselves at the ratio of approximately 1:100. Because there are both positive (desirable) and negative (non-desirable) anomalies, the overall ratio of positive (desirable) anomalies is probably somewhere in the neighborhood of approx. 1:200. Desirable anomalies are very valuable to cannabis breeding providing that they are viable. So always keep an eye out for desirable anomalies and put sufficient energy into their reproduction. More often than not however, anomalies can be very finicky and therefore difficult to work with
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Old 12-03-2006, 03:22 PM   #9 (permalink)
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Part 3

Past Selection Processes Review

Originally, in the late 1970's, I was growing up to 100 plants at a time using over 1000 watts of light, and also outdoors in a backyard garden space. These were all land race sativa that fortunately cloned well. The ratio of highly desirable individuals from these plants was about 1:100. One of the most annoying traits of these varieties was hermaphroditism. Approximately 60% of all of these plants from seed were unmanageable hermis, and about 25% more were what I referred to as manageable hermaphrodites, meaning that with close observation and intense scrutiny the male pods could be seen and eliminated as they appeared. About 15% of these sativa plants were female enough to produce marketable sinsimilla bud, with a constant vigilance toward the occasional stray pollen sack. In other words the hermaphroditism expressed in these equatorial sativa was extreme and nearly total.

A quick word about the virtues of hermaphrodites: Ask any old-time herbalist, one who has been experiencing fine herb since at least the early 1970's, what their favorite all-time herbal variety was, and the answer will be something to the effect of; ?Santa Marta or Acapulco Gold? or ?Highland or Chocolate Thai? or ?Punta Roya (red-tipped gold Highland Oaxacan)? or ?Guerran Green? or ?Panama Red? etc. et. al., all of which were equatorial, or sub-tropical, origin sativa and hermaphroditic. Even the great hashish of the era such as Lebanese Red and Blonde, all Moroccan and Nepalese were produced from seeded stock.

This is not so much in praise of the hermaphrodite as it is a suggestion in regard to the cannabinoid profile of seeded verses non-seeded herb. It has been my experience that the cannabinoid profile of seeded herb produces a wider range of effect than from non-seeded, or sinsimilla, herb. The equatorial environment also probably contributed to a wider range of cannabinoids. One of the aspects of the equatorial environment is its consistent day/night temperature range, there is little difference between day and night temps on the equator supposedly inspiring a wider cannabinoid profile. Couple this with the seeded cannabinoid profile and it becomes easy to understand the popularity of the equatorial produced sativa, despite its hermaphroditic problems. I am curios as to what future research in this capacity may provide.

Once the indica was introduced into the mix the hermaphrodite ?problem? became controllable. It only takes a few zero-tolerance generations indoors to fully eliminate hermaphroditic tendencies. As a matter of fact, this, coupled with shortening the flowering cycle, became the first main concerns of the indoor or commercial horticulturist. This unbalanced focus may be the strongest contributing factor to the ?blandness? of much of the herb to follow. The author ?R? did a cover piece for High Times magazine in the mid-1980's calling for a ?Ban the Bud? campaign, against the indica onslought, due to how bad and bland the quality of some herb was becoming then. I remember the times clearly.

During this period I was beginning to venture out into larger satellite grows (indoor and out) that kicked my selection numbers up to around 1000 plants at a time for awhile. It was from these trials that I was able to do the bulk of my f2 experimentation and selections. I worked with these numbers for enough trials to manipulate and witness the phenomenon of quality production to a high degree of certainty. Once I was certain how to produce the f3's, the f4's and beyond became much easier to produce.

During the late 1980's, and due to the harsh political realities of the times, the high numbers game became too dangerous. The war on some drugs and spooky ops such as Operation Green Merchant forced my experimentation deep underground. Fortunately, the lessons learned prior proved fruitful and progress was possible despite the political weather. I had already learned to produce f3 and f4 Blueberry (et. al.). However, doing so with diminished numbers actually helped boost my learning curve. Between 1987 to 1990 I was able to do so using less than 100 plants from seed at a time. And by 1991 I was able to do adequate selection work from past produced stock using less than 50 plants (seeds) at a time.

Europe

Holland

By the early 1990's I was extremely interested in the burgeoning seed market developing in Holland. I had known about the seed banks since 1983 and was always only interested in obtaining more pure, land-race varieties. Unfortunately, there were only hybrid crosses ever available at the time and I had more than enough of my own to work with. By 1993 I finally made the pilgrimage to Amsterdam where I made new connections. In 1994 I connected with the first company that I worked with in Europe. By 1995 I was supplying this company with seed-stock both for sale and for breed work. I had contracted with this company to produce Blueberry, Flo and Blue Velvet.

The first company I worked with in Europe sprouted only 25 seeds of each of these varieties to make selections from. Other than supplying seed-stock, I was only minimally involved in the selection process. I did get to see the mother and father plants alive, however, the selection process had already been done prior by others. Unfortunately, my relationship with this company was short-lived as all the owner really wanted was my seed-stock. Once he had it I became a very low priority in his scheme. In all honesty I was never paid one red cent for any of the Blueberry (or ?Flow? or Blue Velvet) that company number one in Europe produced (plus having over 3,000 seeds that I produced completely ripped off).

Needless to say this lack of concern prompted me to seek other possibilities that culminated in my relationship with the second company I worked with in Europe. At this company about 50 seeds of each variety were sprouted, but I was once again mainly left out of the selection process except for sampling a number of finished products and making selections based on those (which is enough, actually). I never got to see any of the live plants from this selection process at company number two in Europe. I also contributed seed-stock for three more varieties there; Blue Moonshine, Blue Heaven and Purple Passion. The owner of this company was satisfied with paying me the minimum amount I would consider adequate. Fortunately, part of the deal was my ability to remain independent and work with whomever else I pleased.

Switzerland

The third company I worked with in Europe was in Switzerland. The owner of this company was able to dramatically push the envelope there and some interesting results blossomed. I visited Switzerland three times between 1999 and 2001 and was truly amazed at what I witnessed on each visit. Out of all of the companies that I worked with in Europe, I felt the most involved and productive in Switzerland. I was involved with selections of finished products and with live mother and father plants as well. I even got to help plant, transplant and harvest a few of the gems produced there.

The varieties produced by the third company that I worked with in Europe included Moonshine Rocket Fuel, Rosebud and Blue Satellite. I must admit that the bubble hash from the Blue Satellite is among the finest and most desirable product I have sampled (outside of my own) since the 1980's! Unfortunately, the owner of this company was unable to successfully work with the local authorities and was forced to leave Switzerland. Some truly intrepid tales were spun during the brief stay there and I will remember many of them with delight.

Canada, The True North Strong and Free

With glimmers of hope on the horizon, Canada is fast becoming the Cannabis Breeding capital of the world. With the much-appreciated activism of entrepreneurs such as Marc Emery (et. al.), a new haven for a seriously dedicated cannabis community is developing. One such entrepreneurial dedicate is Red of Legends Seeds. I met Red in Switzerland where he was very busy and involved working for the happening community there. Red is a high-flying, free spirit with a savvy sense of taste.

Red was able to orchestrate the necessary requirements to produce a very large selection process. This grow consisted of about 400 plants (over 200 Blueberry phenos and over 100 Flo). Out of these there ended up being over 160 Blueberry and over 70 Flo females and about 60 males that made the initial cut. Copies of each of these were cloned and meticulously maintained by the crew. This actually turned out to be a slight overkill, but a testimony to the absolute dedication of the crew.
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Part 4

The Crew

Mighty-G is a green-thumbed master gardener whose success with cannabis is phenomenal. Mr. G was able to provide and maintain a near-perfect growing environment for a lengthy period of time as the plants were kept in an extended vegetive state to insure 100% clone success. The plants were absolutely beautiful. Kermit was in charge of clone reproduction and maintenance. Kermit has been a respected part of the local cannabis community for many years. Chimera appeared online a few years ago and has proven himself to be an intelligent and dedicated soul, along with being a focused horticulturist with excellent credentials in the field of genetics. I first learned of Chimera online where he posted to a few message boards that I occasionally lurk and I appreciated the information he shared. The Cannabis Cowboy also added his expertise, especially considering the collection, purification and pressing of the dry-sieved resin.

I just want to give a big ?shout out? and a huge thank you to all of the crew for their very successful efforts on this project. You cats rock! Thank you.

The Process

The main room was divided in two with the Blueberry on the left and the Flo on the right. The plants were relatively huge considering how long they?d been in veg. Lush growth dominated as three distinct Blueberry phenotypes and two distinct Flo presented their development, along with a small number of unique anomalies. Of course, all individuals were numbered and labeled and notes were made over the course of several inspections during the flowering cycle.

During this period all of the males were isolated in a separate room and watched closely to enable the best selection from them. From this particular gene-pool, I find it relatively easy to select the best males as they tend to express their traits regardless of environment or light cycle. There were so many to choose from during this process that the difficulty became who to cull out. Most of the males were at least to some degree resinous with glandular stalked trichome, some more than others. This usually makes it easy to test certain profiles such as overall flavors.

Only after the most desirable males are selected (i.e. all the others rejected) are issues of structure and growth pattern considered. Sweet, fruity and floral expressions are most desirable, but attention is paid to other possibilities as well. Top quality candidates of indica, sativa and mutant anomaly are picked by process of elimination. Then those with the best structure; hollow stems, good color and flower density, become the final candidates.

The females also pose the same problem in regard to who is eliminated. Notes are made as to any outstanding qualifications that present themselves during the bud cycle. But it is not until the sixth week in flower, and sometimes not until the eighth week (or longer if the variety is strongly sativa), that the real differences in individuals becomes apparent and the truly amazing qualities shine. And even then, it only amounts to field-notes until well after harvest and the cut-and-dried product is totally cured. It is then that the final selection process begins.

During our selection-crop numerous individuals could have passed the requirements to be a great mother plant. By and large, the overall ratio of desirable plants that qualified for final selection from this crop was approximately 1:10 (employing DJ?s Law). As it turns out the elite ratio of final candidates turned out to be approximately 1:30?the best of the best as it were. By the eighth week in bud approximately two dozen individuals stood out as primary candidates. After these samples were individually labeled and jar cured for about two months, a total of eleven were of supreme quality. Believe it or not, the final elimination process among these eleven was perhaps the most difficult to complete. Part of the sprocess involved selecting one of each of the three Blueberry phenotypes, one of the Flo, one Blue Moonshine and deciding on the possibility of something new.


The Varieties

After the fourth week in bud, generally speaking, certain characteristics become apparent. On the Blueberry side of the room three distinct phenotypes presented themselves, while on the flo side two less distinct phenos appeared. The three Blueberry phenotypes could be referred to as indica, sativa and variegated or mutated. The indica were shorter, denser and had larger calyx and bract leafs making the buds look plump. The sativa were taller, more slender leafed with more elongated buds of dense, smaller calyx. The indica tended to be of a stronger, more musky odor where the sativa were more delicate and floral. The variegated or mutated individuals varied more in their aromatic palate with some seeming more potent than others. On the flo side the difference was less pronounced between phenotypes but two distinct types developed. The primary difference was in bud structure and formation with one type growing with its bract leaves pointing more up and the other with its bract leaves pointing down. Both were more sativa looking with dense buds of small calyx. There was also a difference in potency of aroma between these individuals.

The seed stock ?True Blueberry? currently under scrutiny derived from f2?s that were very ?BK? or Berry Kush-like. These f2 ?BK??s were crossed with very ?TF?, or ?True Floral?, sometimes referred to as ?Temple Flo?, mates in the f3 and/or f4 generation to brighten the head considerably. Once the right mix was discovered these f4?s (and beyond) crosses were inline bred (filial crossed) to stabilize the proper traits. The ?flo? pheno?s are closer to the ?TF? (?True Floral?, ?Temple Flo?), headier side of the mix, most reminiscent of the Highland Oaxaca Gold.



?Grape Krush? (or ?Blue Krush?)?a productive, deep-colored hybrid of very high quality. This plants exhibits partial to full leaf-deformities of the ?krinkle? type, but with good structure and heavy bud production of large calyxes. The buds express a strong sharp/fruity odor with a distinct sweet/grape flavor brought out in the cure. A strong, long-lasting head/body mix is evident in the finished product with an exciting, but not ?racy?, head and a mild narcotic body. Very euphoric and desirable effects that most seasoned connoisseurs prefer. 50-60 day flowering time.

?Flodica? ? a mostly indica phenotype from the flo line. A rare, near-total recessive indica found by chance in the ?TF? line (?TF?= ?Temple Flo? or ?True Floral?). Generally, the flo line sports very sativa like structures of taller plants with slender leaves and spear-shaped buds. The ?Flodica?, however, is a near-pure indica phenotype of short, stout, yet productive, structure with very large, dense, dark indica buds. Very resinous with heavy gland production of an earthen palate to the buds that produce a very strong, narcotic-type experience. 50-55 day flowering time. Unfortunately, the ?Flodica? (and the ?True Blue Moonshine?) were nearly sterile--i.e. no (or very few) seeds developed, and were therefore culled.

?True Blueberry??the ultimate hybrid of Blueberry expression. Selected for its superior quality from a large pool, this hybrid contains the best from both worlds (indica and sativa). Medium height with long, fruity and productive buds of medium sized calyxes. Beautiful lavender hues become apparent soon into the flowering cycle. The finished product is of the highest quality with sweet, elongated Blueberry buds destined to please the most finicky palate. High resin production as expected from the ?Blue? family. 50-60 days flowering time.

?True Blue Moonshine??a true ?hash-plant?. Selected for its outstanding production of large, clear gland heads, this mostly-indica hybrid really packs a musky/fruity punch. Medium height producing parge, dense buds glistening with trichomes. More musky than fruity with a burgundy/earthen flavor at cure. Top-notch Moonshine. 50-60 days flowering time.

?F-13"?a Holy Grail plant of four-star excellence. Previously unreleased, a very desirable product and potential breeder. A more-sativa hybrid of medium height with long, spear-shaped, dense and resinous buds and an earlier finish time than most sativa. The superfluous quality of the finished product is remarkable: a clear, clean, crisp head of the kindest order with a sweet/floral flavor. This girl really rings the bell every time! Not for the couch-lock crowd, this heady sativa is for those who truly enjoy its stimulating yet comfortable appeal. A real day (or night) brightener. My personal favorite from this batch. 50-65 days flowering time.

Stay tuned for future re-releases of Velvet Luna (formerly Blue Satellite and Blueberry Sativa), Moonshine Rocket Fuel and Rosebud in the not-too-distant future. Have fun and best regards toward your horticultural ventures. Enjoy!
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Old 12-04-2006, 05:29 AM   #11 (permalink)
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Thanks Dan, this is gold. DJ is such a freak.
I like how he describes himself as more of an artist than a scientist.
I've been thinking of searching for his articles, thanks for posting these marvels.
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Old 12-04-2006, 06:20 PM   #12 (permalink)
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If you run across anymore post em up please.
He takes a very spiritual approach to his weed. I forget who it was that takes chemicals with his pot to judge it.
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Old 02-02-2008, 10:19 AM   #13 (permalink)
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There's so much more GOOD information on growing online than in ANY single grow book.
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