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Old 01-10-2018, 08:33 PM   #50821 (permalink)
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Probably. Working with my prof would be the wisest decision if im thinking career path.
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Old 01-11-2018, 04:39 AM   #50822 (permalink)
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if you can get away with it do your own thing

i would not make a good career counsellor
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Old 01-11-2018, 04:55 AM   #50823 (permalink)
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running your own project from start to finish will give you lots of skills you won't get from working on someone elses project
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Old 01-11-2018, 11:26 AM   #50824 (permalink)
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Yes having oversight and control (sort of) over an experiment that I find personally interesting was the deciding factor


Plus this will be more challenging.
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Old 01-11-2018, 11:52 AM   #50825 (permalink)
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It's 40F degrees here right now. Suppose to be 10F degrees tomorrow. Old people talk about the weather alot.
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Old 01-11-2018, 12:35 PM   #50826 (permalink)
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Quote:
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Yes having oversight and control (sort of) over an experiment that I find personally interesting was the deciding factor


Plus this will be more challenging.
hit me up with any questions if you get bogged down by the orbitals
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Old 01-11-2018, 12:36 PM   #50827 (permalink)
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the most useful thing sometimes is if you are in a rut is to try and explain your confusion to a bunch of people with different backgrounds
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Old 01-11-2018, 01:48 PM   #50828 (permalink)
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Quote:
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It's 40F degrees here right now. Suppose to be 10F degrees tomorrow. Old people talk about the weather a lot.
Thanks a lot, now I hafta talk about it.
Yesterday it rained a lot almost 3" in 24 hours. You have to remember it rains slightly less than 10" a year here, so 3" is a big deal. And the last 2 weeks its been getting down into the high 50's, few nites the low 60's. I live up on a hill so the mud-slides arent affecting my local, but rain is raising hell in the burn areas.

After I posted 'bout my arm, no one posted in random for a few days, I thought I grossed you all out, too much detail.

-c-
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Old 01-11-2018, 02:06 PM   #50829 (permalink)
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Can you think of a way to safely alter the pH of an Anthocyanin sample without introducing something that could inhibit photon capture and subsequent electron transfer in the dye to TiO2 interface?
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Old 01-11-2018, 02:10 PM   #50830 (permalink)
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If not, no big deal because I'm going to cover my bases. I'm gonna order:
Anthocyanin from as many sources I can find/afford
Carotenoids , ditto
Chlorophyl A and B


That should cover all the visible spectrum I think
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Old 01-11-2018, 02:59 PM   #50831 (permalink)
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Quote:
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Can you think of a way to safely alter the pH of an Anthocyanin sample without introducing something that could inhibit photon capture and subsequent electron transfer in the dye to TiO2 interface?
Centrifugal diafiltration through desalting filter?
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Old 01-11-2018, 04:08 PM   #50832 (permalink)
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Hey Kishar
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Old 01-11-2018, 04:09 PM   #50833 (permalink)
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@kitch: that sounds like it does pH one way


But anyway I think it's a pointless question now, there are many sources of the dye so I prolly won't have to do anything of the sort
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Old 01-11-2018, 07:13 PM   #50834 (permalink)
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Quote:
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@kitch: that sounds like it does pH one way


But anyway I think it's a pointless question now, there are many sources of the dye so I prolly won't have to do anything of the sort
It actually just removes all buffer while leaving your desired reagent, you'd need a very fine filter but you should be able to set the pH of your dye to whatever you want then spin it down to remove everything but the pigment.
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Old 01-12-2018, 01:00 AM   #50835 (permalink)
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we used frozen raspberries and ethanol to extract the dye

probably the best lab recipe ever. like making a cocktail
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Old 01-12-2018, 01:06 AM   #50836 (permalink)
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to change the pH just use burets and titration. not sure how it would affect the experiment
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Old 01-12-2018, 01:10 PM   #50837 (permalink)
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I'm gonna need me some ethanol then cuz it looks like I will end up having to extract myself.


Getting the dyes on their own is looking pretty damn expensive

In fact I'm having trouble finding anything that's just a dye that's already been extracted, which isn't part of some other product.
A lot is just like.. pure powder with some percentage of anthocyanin... which would require extraction anyway so...

Ethanol and a trip to the grocery store
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Old 01-12-2018, 03:27 PM   #50838 (permalink)
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frozen raspberries. should cost a couple of dollars. denatured ethanol you probably have already in some lab

do you need to extract it in its pure form for your idea? thats also easy (for chemists i guess)
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Old 01-12-2018, 05:02 PM   #50839 (permalink)
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From what I gathered in the paper you sent me, the extraction was super basic, and the stuff they used to stain the cell was pretty far from 'pure'


Ideally though it's as pure as possible though since for the theory side I'm going to propose a model that uses a pure sample, ie no consideration of stray proteins and sugars hanging around and mucking things up.

That said, initially I'm going to be doing probably whatever is easiest/quickest so I can at least establish that I can successfully build a working DSSC
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Old 01-12-2018, 05:40 PM   #50840 (permalink)
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It'd be cool to teach ion exchange chromatography with a mixed sample of anthocyanins at different pH because it will give a great visual representation of fractionation...

Proteins would be really easy to filter out because they'll be much much larger than your desired product.
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